Differential Interference Contrast (DIC)

Differential Interference Contrast (DIC)

General remarks

  • DIC allows observation of unstained specimens by generation of image contrast
  • Structures of interest must have different refractive indices (r.i.).

Beam path for DIC microscopy

Keep in mind: there are dedicated DIC prism / objective combinations. Check appropriate combinations from the Objective list of the manual or the Facility web site.

1. Upright microscope in laser scanning mode (from top to bottom)

  • Light source (laser)
  • No Polarizer (laser has to provide correctly polarized light)
  • DIC prism at objective
  • Objective
  • Sample
  • Condenser front lens
  • DIC prism (condenser)
  • Analyzer (polarizing filter)
  • Transmission detector (PMT)

2. Inverted microscope in halogen transillumination mode (from top to bottom)

  • light source (halogen lamp)
  • Polarizer (polarizing filter)
  • DIC prism (condenser)
  • Condenser front lens
  • Sample
  • Objective
  • DIC prism at objective
  • Analyzer (polarizing filter)
  • Detector (camera, eye)

3. Upright microscope in halogen transillumination mode (from top to bottom)

  • Detector (camera, eye)
  • Analyzer (polarizing filter)
  • DIC prism at objective
  • Objective
  • Sample
  • Condenser front lens
  • DIC prism (condenser)
  • Polarizer (polarizing filter)
  • light source (halogen lamp)

4. Inverted microscope in laser scanning mode (from top to bottom)

  • Transmission detector (PMT)
  • Analyzer (polarizing filter)
  • DIC prism (condenser)
  • Condenser front lens
  • Sample
  • Objective
  • DIC prism at objective
  • no Polarizer (laser has to provide correctly polarized light)
  • light source (laser)
 
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