Mikroskope

LEICA TCS SP5 confocal microscope

  • upright microscope stand
  • Ar Laser (457, 476, 488,514 nm)
  • DPSS Laser (561 nm)
  • HeNe Laser (633 nm)
  • standard scanner and high speed resonant scanner
  • Acousto Optical Beam Splitter (AOBS) as main beam splitter for free selection of and rapid switching between reflected / transmitted wavelengths
  • Spectral scanner with adjustable windows for fluorochromes showing blue to dark red fluorescence and for different Green Fluorescent Protein Variants (CFP, GFP, YFP) and dsRed
  • 4 PMTs (epifluorescence and reflection mode)
  • One PMT (transmission mode)
  • Maerzhaeuser XY stage (manual and software-controlled mode)
  • Software for sequential / simultaneous / time lapse / spectral recording / and for photobleaching experiment

LEICA TCS SP2 confocal microscope

  • two microscope stands (upright, inverted)
  • Ar UVLaser (352, 364 nm)
  • Ar Laser (457, 476, 488,514 nm)
  • HeNe Laser (543 nm)
  • HeNe Laser (633 nm)
  • Acousto Optical Beam Splitter (AOBS) as main beam splitter for free selection of and rapid switching between reflected / transmitted wavelengths
  • Spectral scanner with adjustable windows for fluorochromes showing blue to dark red fluorescence and for different Green Fluorescent Protein Variants (CFP, GFP, YFP) and dsRed
  • 4 PMTs (epifluorescence and reflection mode) One PMT (transmission mode)
  • One transmission detector (W. Denk design)
  • Maerzhaeuser XY stage (manual and software-controlled mode)
  • Software for sequential / simultaneous / time lapse / spectral recording / and for photobleaching experiment

LEICA TCS NT confocal microscope

  • Ar/Kr Laser (475, 488,568 and 647 nm)
  • HeCd Laser (442 nm)
  • Filter sets for common fluorochromes (cyan to dark red fluorescence) and different Green Fluorescent Protein Variants (CFP, GFP, YFP)
  • 3 PMTs (epifluorescence and reflection mode)
  • 1 PMT (transmission mode)
  • Software for sequential / simultaneous / time lapse recording / and for photobleaching experiments

LEICA MZ FL 3 stereo microscope

  • Mercury lamp and Schott cold light guide lamp
  • Filter sets for common fluorochromes (DAPI LP, CFP BP, EGFP LP, EGFP BP, YFP BP, dsRed LP)
  • LEICA DC 350 FX b/w XGA CCD camera

ZEISS LSM 510 META NLO multiphoton / confocal microscope

  • Upright stand (ZEISS Axioskop 2 FS MOT on a LUIGS+NEUMANN xy stage)
  • COHERENT Chameleon Ultra II Ultrashort Pulse Laser (fs pulses, 690 - 1080 nm)
  • Ar Laser (457, 476, 488, 514 nm)
  • HeNe Lasers (543 and 633 nm)
  • Filter sets for common fluorochromes (cyan to dark red fluorescence) and different Green Fluorescent Protein Variants (CFP, GFP, YFP)
  • META detector (PMT array)
  • 3 PMTs (epifluorescence and reflection mode)
  • One PMT (transmission mode, also used for transmission NDD, see below)
  • 2 sets of non-descanned detectors (NDDs) for epifluorescence and transmission fluorescence mode
  • Software for sequential / simultaneous / time lapse recording / and for photobleaching experiments
  • Iontophoresis system (npi MVCS-02C)
  • Two micromanipulators (LUIGS+NEUMANN)
  • Electrophysiological recording equipment (HEKA EPC 9 plus Software on Apple Macintosh)

ZEISS Axiovert 200M Live Imaging system

  • motorized, inverted microscope
  • CO2 and temperature controlled environment chamber (EMBLEM)
  • TILL Photonics Polychrome V monochromator or ZEISS Hg lamp
  • Filter sets for common fluorochromes (DAPI to Cy5)
  • Filter sets for Ca imaging (Fura-2)
  • PCO Sensicam QE cooled CCD camera
  • Maerzhaeuser motorized, software-controlled XY stage
  • QED In VIVO image recording software

ZEISS Axiovert 135 inverted microscope

  • motorized, inverted microscope
  • Mercury lamp Filter sets for common fluorochromes (blue to dark red fluorescence)
  • Maerzhaeusser motorized, software-controlled XY stage
  • Sensicam Image recording and processing software
  • PCO Sensicam QE cooled CCD camera

VISITECH QLC100 spinning disc confocal microscope with EM-CCD camera / DualView

  • Coherent C70 Spectrum ArKr laser (457, 488, 514, 568, 647 nm)
  • LEICA DM-RXA2 microscope Wide field filter sets for common fluorochromes (DAPI to Texas Red)
  • software-controlled Z stage
  • QED In VIVO image recording software
  • Media Cybernetics ImagePro Analyzer image analysis software
  • DuaView for side-by-side recording of two fluorescence signals (green/red or cyan/yellow)
  • Roper Scientific Cascade II EM-CCD for fluorescence recording
  • Retiga b/w high resolution camera for DIC recording)
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