Mikroskope

ZEISS LSM 510 META NLO multiphoton / confocal microscope

  • Upright stand (ZEISS Axioskop 2 FS MOT on a LUIGS+NEUMANN xy stage)
  • COHERENT Chameleon Ultra II Ultrashort Pulse Laser (fs pulses, 690 - 1080 nm)
  • Ar Laser (457, 476, 488, 514 nm)
  • HeNe Lasers (543 and 633 nm)
  • Filter sets for common fluorochromes (cyan to dark red fluorescence) and different Green Fluorescent Protein Variants (CFP, GFP, YFP)
  • META detector (PMT array)
  • 3 PMTs (epifluorescence and reflection mode)
  • One PMT (transmission mode, also used for transmission NDD, see below)
  • 2 sets of non-descanned detectors (NDDs) for epifluorescence and transmission fluorescence mode
  • Software for sequential / simultaneous / time lapse recording / and for photobleaching experiments
  • Iontophoresis system (npi MVCS-02C)
  • Two micromanipulators (LUIGS+NEUMANN)
  • Electrophysiological recording equipment (HEKA EPC 9 plus Software on Apple Macintosh)

ZEISS Axiovert 200M Live Imaging system

  • motorized, inverted microscope
  • CO2 and temperature controlled environment chamber (EMBLEM)
  • TILL Photonics Polychrome V monochromator or ZEISS Hg lamp
  • Filter sets for common fluorochromes (DAPI to Cy5)
  • Filter sets for Ca imaging (Fura-2)
  • PCO Sensicam QE cooled CCD camera
  • Maerzhaeuser motorized, software-controlled XY stage
  • QED In VIVO image recording software

ZEISS Axiovert 135 inverted microscope

  • motorized, inverted microscope
  • Mercury lamp Filter sets for common fluorochromes (blue to dark red fluorescence)
  • Maerzhaeusser motorized, software-controlled XY stage
  • Sensicam Image recording and processing software
  • PCO Sensicam QE cooled CCD camera
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