BMM Seminar with Michael Heymann - Nano 3D printed microfluidics to understand biological dynamics across scales
BMM Seminar with Michael Heymann - Nano 3D printed microfluidics to understand biological dynamics across scales
- Date: Oct 22, 2018
- Time: 12:30 PM - 01:15 PM (Local Time Germany)
- Speaker: Dr. Michael Heymann
- Max Planck Institute of Biochemistry, Martinsried
- Location: MPI for Medical Research
- Room: Seminar Room A/B
- Host: Prof. Dr. Ilme Schlichting
Abstract:
Two photon stereolithography is a sub-micron precise 3D
printing technique that allowed us to overcome
long-standing challenges in microfluidic engineering for
applications ranging from time-resolved serial crystallography
to synthetic biology.
To understand enzyme catalysis and protein
conformational changes at the atomic scale, we pioneered
novel ultracompact microfluidics for time-resolved structural
biology to record ‘molecular movies’ of substrate turn-over.
This method allows to determine the structures of transient
states and thereby kinetic mechanisms. We could follow
the catalytic reaction of the M. tuberculosis β-lactamase with
the 3rd generation antibiotic ceftriaxone with millisecond to
second time resolution at 2 Å spatial resolution.
We furthermore achieved fast jets exceeding 100 m/s for
megahertz serial crystallography at the European XFEL.
In extending this technology to synthetic biology, we
can reconstitute functional biological and biomimetic systems
from the bottom up with unprecedented precision and
throughput. For instances to compartmentalize the E.coli MinDE
protein oscillator, that positions the cell division machinery
at mid-cell, into physiologically relevant three-dimensional
model compartments, such as lipid vesicles.
In current efforts, we are developing novel protein
photoresists to nano-3D-print sub-cellular compartments with
the highest achievable functional conformity to cellular
structures in vivo. In first
proof-of-principle experiments we structured a contractile
eukaryotic cell division model. Such in vitro model
systems will allow to resolve dynamic biological states far
from equilibrium to study the hierarchical assembly of active
bio-materials, to resolve basic principles of synchronization,
morphogenesis and differentiation in confined geometries, as
well as for applications in biochemical information processing
in the future.