Choosing fluorochromes and fluorochrome combinations

Choosing fluorochromes and fluorochrome combinations

Single labeling

  • signal strength
  • background
  • suitable excitation sources (Hg lines, laser lines)
  • suitable detection filter combinations (also dependent on excitation source)

Dual or triple labeling

  • relative signal strength
  • possible FRET (fluorescence resonance energy transfer), if emission spectrum of a fluorochrome overlaps with excitation spectrum of another fluorochrome in use and, in addition, the distance between both is <10 nm)
 
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