Barends, Thomas R. M.
Thomas R. M. Barends
Phone: +49 6221 486-508
Fax: +49 6221 486-585
Fuhry, Elisabeth
Elisabeth Fuhry
Phone: +49 6221 486-650
Room: R. 026a


Jahn, B.; Pol, A.; Lumpe, H.; Barends, T.; Dietl, A.; Hogendoorn, C.; den Camp, H. J. M. O.; Daumann, L. J.: Similar but not the same:first kinetic and structural analyses of a methanol dehydrogenase containing a europium ion in the active site. ChemBioChem: A European Journal of Chemical Biology 19 (11), pp. 1147 - 1153 (2018)

Structural analysis of a protein in which the usual rare earth cofactor is replaced with europium

Determination of structure and kinetics reveals the effects  of several  different rare earths on catalytic activity.

July 09, 2018

Most organisms depend on commonly occurring substances, but some bacteria use rare earth elements (REEs) such as lanthanum, cerium or europium as cofactors of metalloenzymes - enzymes that catalyze chemical reactions with the help of a metal ion. One of these is a methanol dehydrogenase (MDH), an enzyme used by the bacteria in harvesting energy. Scientists at the Max Planck Institute for Medical Research in Heidelberg and their collaborators* have now determined the structure and kinetics of an MDH with a europium ion as cofactor in the active site, the first ever example of an enzyme using this element. By comparison with previous analyses, they were able to establish that while the enzyme is 200 times slower than with other rare earth elements the overall structure remains the same. This lower activity must therefore be the result of very subtle changes active site chemistry. The observed variations in the protein’s efficiency in catalyzing the chemical reaction for which it is required, probably reflect the adaptation of the bacteria to the lighter REEs, which are more abundant in the environment.

The cofactor europium at the active site of the protein MDH. Zoom Image
The cofactor europium at the active site of the protein MDH.

* from the Ludwig-Maximilians-Universität in Munich and the Institute for Water and Wetland Research in Nijmegen

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