Microscopes

    Microscopes

    LEICA TCS SP5 confocal microscope

    • upright microscope stand
    • Ar Laser (457, 476, 488,514 nm)
    • DPSS Laser (561 nm)
    • HeNe Laser (633 nm)
    • standard scanner and high speed resonant scanner
    • Acousto Optical Beam Splitter (AOBS) as main beam splitter for free selection of and rapid switching between reflected / transmitted wavelengths
    • Spectral scanner with adjustable windows for fluorochromes showing blue to dark red fluorescence and for different Green Fluorescent Protein Variants (CFP, GFP, YFP) and dsRed
    • 4 PMTs (epifluorescence and reflection mode)
    • One PMT (transmission mode)
    • Maerzhaeuser XY stage (manual and software-controlled mode)
    • Software for sequential / simultaneous / time lapse / spectral recording / and for photobleaching experiment

    LEICA TCS SP2 confocal microscope

    • two microscope stands (upright, inverted)
    • Ar UVLaser (352, 364 nm)
    • Ar Laser (457, 476, 488,514 nm)
    • HeNe Laser (543 nm)
    • HeNe Laser (633 nm)
    • Acousto Optical Beam Splitter (AOBS) as main beam splitter for free selection of and rapid switching between reflected / transmitted wavelengths
    • Spectral scanner with adjustable windows for fluorochromes showing blue to dark red fluorescence and for different Green Fluorescent Protein Variants (CFP, GFP, YFP) and dsRed
    • 4 PMTs (epifluorescence and reflection mode) One PMT (transmission mode)
    • One transmission detector (W. Denk design)
    • Maerzhaeuser XY stage (manual and software-controlled mode)
    • Software for sequential / simultaneous / time lapse / spectral recording / and for photobleaching experiment

    LEICA TCS NT confocal microscope

    • Ar/Kr Laser (475, 488,568 and 647 nm)
    • HeCd Laser (442 nm)
    • Filter sets for common fluorochromes (cyan to dark red fluorescence) and different Green Fluorescent Protein Variants (CFP, GFP, YFP)
    • 3 PMTs (epifluorescence and reflection mode)
    • 1 PMT (transmission mode)
    • Software for sequential / simultaneous / time lapse recording / and for photobleaching experiments

    LEICA MZ FL 3 stereo microscope

    • Mercury lamp and Schott cold light guide lamp
    • Filter sets for common fluorochromes (DAPI LP, CFP BP, EGFP LP, EGFP BP, YFP BP, dsRed LP)
    • LEICA DC 350 FX b/w XGA CCD camera

    ZEISS LSM 510 META NLO multiphoton / confocal microscope

    • Upright stand (ZEISS Axioskop 2 FS MOT on a LUIGS+NEUMANN xy stage)
    • COHERENT Chameleon Ultra II Ultrashort Pulse Laser (fs pulses, 690 - 1080 nm)
    • Ar Laser (457, 476, 488, 514 nm)
    • HeNe Lasers (543 and 633 nm)
    • Filter sets for common fluorochromes (cyan to dark red fluorescence) and different Green Fluorescent Protein Variants (CFP, GFP, YFP)
    • META detector (PMT array)
    • 3 PMTs (epifluorescence and reflection mode)
    • One PMT (transmission mode, also used for transmission NDD, see below)
    • 2 sets of non-descanned detectors (NDDs) for epifluorescence and transmission fluorescence mode
    • Software for sequential / simultaneous / time lapse recording / and for photobleaching experiments
    • Iontophoresis system (npi MVCS-02C)
    • Two micromanipulators (LUIGS+NEUMANN)
    • Electrophysiological recording equipment (HEKA EPC 9 plus Software on Apple Macintosh)

    ZEISS Axiovert 200M Live Imaging system

    • motorized, inverted microscope
    • CO2 and temperature controlled environment chamber (EMBLEM)
    • TILL Photonics Polychrome V monochromator or ZEISS Hg lamp
    • Filter sets for common fluorochromes (DAPI to Cy5)
    • Filter sets for Ca imaging (Fura-2)
    • PCO Sensicam QE cooled CCD camera
    • Maerzhaeuser motorized, software-controlled XY stage
    • QED In VIVO image recording software

    ZEISS Axiovert 135 inverted microscope

    • motorized, inverted microscope
    • Mercury lamp Filter sets for common fluorochromes (blue to dark red fluorescence)
    • Maerzhaeusser motorized, software-controlled XY stage
    • Sensicam Image recording and processing software
    • PCO Sensicam QE cooled CCD camera

    VISITECH QLC100 spinning disc confocal microscope with EM-CCD camera / DualView

    • Coherent C70 Spectrum ArKr laser (457, 488, 514, 568, 647 nm)
    • LEICA DM-RXA2 microscope Wide field filter sets for common fluorochromes (DAPI to Texas Red)
    • software-controlled Z stage
    • QED In VIVO image recording software
    • Media Cybernetics ImagePro Analyzer image analysis software
    • DuaView for side-by-side recording of two fluorescence signals (green/red or cyan/yellow)
    • Roper Scientific Cascade II EM-CCD for fluorescence recording
    • Retiga b/w high resolution camera for DIC recording)
     
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