Forschungsgruppen Leiter

Schlichting, Ilme
Ilme Schlichting
Telefon: +49 6221 486-500
Fax: +49 6221 486-585
Reinstein, Jochen
Jochen Reinstein
Telefon: +49 6221 486-502
Fax: +49 6221 486-585
Barends, Thomas R. M.
Thomas R. M. Barends
Telefon: +49 6221 486-508
Fax: +49 6221 486-585
Doak, R. Bruce
R. Bruce Doak
Telefon: +49 6221 486-267
Fax: +49 6221 486-585
Fischer, Matthias
Matthias Fischer
Telefon: +49 6221 486-506
Fax: +49 6221 486-585


Shoeman, Robert L.
Robert L. Shoeman
Telefon: +49 6221 486-577
Fax: +49 6221 486-585
Domratcheva, Tatiana
Tatiana Domratcheva
Telefon: +49 6221 486-504
Fax: +49 6221 486-585



Coherent Diffractive Imaging: Ilme Schlichting

Structural biology, and in particular scattering based techniques making use of X-rays and electrons, has provided high-resolution insight in the structure and function of molecules, molecular assemblies, and cells. Despite a lot of advances in instrumentation, radiation damage limits high resolution imaging of biological material using conventional X-ray or electron based approaches. X-ray free-electron lasers (FELs) exceed the peak brilliance of conventional synchrotrons by almost 10 billion times. They promise to break the nexus between radiation damage, sample size, and resolution by providing extremely intense femtosecond X-ray pulses that pass the sample before the onset of significant radiation damage.


Photoreceptors: Ilme Schlichting

Since light is an important environmental variable, many organisms have evolved signalling pathways that transmit and thereby translate this stimulus into various biochemical activities. Recently, new classes of blue light photoreceptors have been identified that use flavin based photosensors. The photosensor domains are coupled to an array of other domains, including kinases and transcription factors. We are mainly studying proteins containing LOV and BLUF domains as photosensors using structural (diffraction, scattering), biochemical and in collaboration quantum chemical approaches. Together with spectroscopic data, their combination allows to understand on a molecular level how absorption of a blue light photon results in a specific structural change of the protein that triggers a secondary signal resulting in a biological event (see e.g. Nature 459: 1015-1018 (2009)). We aim to exploit this knowledge to design new photoreceptors for application in cell biology (see e.g. Nature 461: 104-108 (2009)). [mehr]

Molecular Chaperones: Jochen Reinstein

Attaining a well defined three dimensional structure and thus functionality can be a serious challenge in the early life of many proteins. Although the final structure is energetically favored, many side reactions can occur mostly leading to aggregation that prevent the formation of the native protein structure. Molecular chaperones are ubiquitous in prokarytic/eukaryotic organisms and form cellular networks which assist protein folding in the cell. [mehr]

Strukturbiologie von Elementzyklen: Thomas Barends

The discovery of anammox bacteria in the 1990's has dramatically changed our understanding of the global nitrogen cycle. These bacteria perform ANaerobic AMMonium Oxidation (ANAMMOX), combining ammonium with nitrite into molecular dinitrogen (N2) and water, yielding energy for the cell. This process relies on highly unusual intermediates such as hydrazine. We are studying the molecular mechanism of the ANAMMOX process using structural biology. [mehr]

Invention and Engineering of Sample Delivery Techniques for Advanced X-ray Sources: R. Bruce Doak

ACTIVITIES.  Bruce Doak and his group invent and develop novel methods of sample delivery for use at advanced X-ray sources, including X-ray Free-Electron Lasers (XFEL) and fourth generation synchrotrons.  Based on their research and development, they design and fabricate well-engineered sample injectors for X-ray scattering facilities worldwide.

Viruses of Protists: Matthias Fischer

Viruses are the most abundant biological entities on the planet and have actively influenced the evolution of life since its very beginnings. Whereas viruses as pathogens of humans and livestock have been intensely studied for a century, much less is known about the majority of viruses – especially those that infect microbes. Protists are unicellular eukaryotes and harbor a wide spectrum of viruses, from small RNA viruses to giant DNA viruses. [mehr]

Analytical Protein Biochemistry: Robert Shoeman

The performance of instrumentation for the precise biochemical characterization of proteins has increased dramitically in recent years, primarily as a result of improvements in computer hardware. This is particularly obvious in the field of mass spectrometry, where newer instruments show increases in resolution and sensitivty of more than 1000-fold over instruments dating from the year 2000, and in robotics, where sophisticated as well as dedicated instruments have become affordable and usable, even for small groups of users. [mehr]

Computational Photobiology: Tatjana Domratcheva

Sunlight is an important environmental factor and light-induced chemical reactions may have both beneficial and detrimental biological effects. [mehr]
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